Vesículas extracelulares como predictores tempranos de diabetes gestacional

Autores/as

  • Mario Arias Universidad de los Andes
  • Lara J Monteiro Universidad de los Andes
  • Stephanie Acuña-Gallardo Universidad de los Andes
  • Manuel Varas-Godoy Universidad de los Andes
  • Gregory E Rice Universidad de los Andes
  • Max Monckeberg Universidad de los Andes
  • Pilar Díaz Universidad de los Andes
  • Sebastián E Illanes Universidad de los Andes

Palabras clave:

Biomarkers, Diabetes, Gestational, Extracellular Vesicle

Resumen

Background: During pregnancy, there is an increase in the amount of extracellular vesicles, especially placental exosomes, in maternal plasma. Aim: To isolate and characterize extracellular vesicles from blood during the three trimesters of pregnancy and to evaluate their capacity to identify patients at risk of developing gestational diabetes. Material and methods: A case-control study was conducted in a cohort of 50 pregnant women with plasma samples taken in each trimester. Six women who developed gestational diabetes were paired with three healthy controls per case (a total of 19). Clinical characteristics were recorded at first prenatal appointment, and blood samples were obtained during the first, second and third trimesters. Extracellular vesicles were isolated from plasma by the commercial kit, ExoQuick™. Nanoparticle tracking analysis, was used to characterize the obtained extracellular vesicles. Results: The total concentration of extracellular particles isolated from maternal plasma increased along with gestational age. The size of the extracellular vesicles obtained in the first trimester of pregnancy was very similar between groups (144 ± 37 nm for controls and 143 ± 34 nm for patients with gestational diabetes mellitus). Moreover, the concentration of extracellular vesicles collected in the first trimester, was significantly higher in patients who developed gestational diabetes mellitus later in pregnancy compared to normoglycemic pregnant women (7.94 x108 and 5.15 x108, p = 0.03). Conclusions: Our results provide an insight into the potential capacity of first trimester plasma extracellular vesicles as early biomarkers for the prediction of gestational diabetes mellitus.

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Publicado

2019-11-28

Cómo citar

Arias, M., Monteiro, L. J., Acuña-Gallardo, S., Varas-Godoy, M., Rice, G. E., Monckeberg, M., Díaz, P., & Illanes, S. E. (2019). Vesículas extracelulares como predictores tempranos de diabetes gestacional. Revista Médica De Chile, 147(12). Recuperado a partir de https://mail.revistamedicadechile.cl/index.php/rmedica/article/view/7689

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Sección

Artículos de Investigación