Diferenciación del complejo Entamoeba histolytica/Entamoeba dispar mediante GAL/GALNAC-LECTINA y PCR en aislamientos colombianos.

Autores/as

  • Omaira Yaneth López Universidad Nacional de Colombia.
  • Myriam Consuelo López Universidad Nacional de Colombia
  • Vladimir Corredor Universidad Nacional de Colombia
  • María Clara Echeverry Universidad Nacional de Colombia
  • Análida Elizabeth Pinilla Universidad Nacional de Colombia.

Palabras clave:

Dhg12, Entamoeba dispar, Entamoeba histolytica, Galectins

Resumen

DIFFERENTIATION OF ENTAMOEBA HISTOLYTICA FROM ENTAMOEBA DISPAR USING GAL/GALNAC-LECTIN AND POLYMERASE CHAIN REACTION

Background: Entamoeba histolytica and Entamoeba dispar are morphologically identical. However, the former is highly pathogenic and the latter is not. Aim: To differentiate Entamoeba histolytica from Entamoeba dispar through ELISA and PCR techniques in Colombian isolates from feces. Material and methods: Descriptive study of  Colombian fecal samples from 53 males and 47 women, that were positive for the complex E. histolytica/E. dispar on light microscopy. Positive samples were cultured on Robinson medium to isolate trophozoites. The presence of specific  Gal/GalNAc-lectin was determined by ELISA and polymerase chain reaction in genomic DNA, using the combination of three nucleotides that recognize a variable region of 16S small subunit ribosomal RNA, generating a 166 base pair (bp) product for E. histolytica and 752 pb product for E. dispar. Results: After verification, only eight of the 100 samples were positive for the complex E. histolytica/E. dispar and were cultivated. Isolates were obtained in six cultures, one corresponded to E. histolytica  and six to E. dispar. Conclusions: The presence of E. histolytica/E. dispar complex was largely overestimated with light microscopy. In the few samples where isolates were obtained, the technique described differentiated between both strains.

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Biografía del autor/a

Omaira Yaneth López, Universidad Nacional de Colombia.

Universidad Nacional de Colombia. Carrera 30 # 45 - 03. Bogotá. Colombia. 57 (1) 3165405.

Myriam Consuelo López, Universidad Nacional de Colombia

Universidad Nacional de Colombia. Carrera 30 # 45 - 03. Bogotá. Colombia. 57 (1) 3165405. Profesora Asociada. Departamento de Salud Pública. Facultad de Medicina.

Vladimir Corredor, Universidad Nacional de Colombia

Universidad Nacional de Colombia. Carrera 30 # 45 - 03. Bogotá. Colombia. 57 (1) 3165405. Profesor Asociado. Departamento de Salud Pública. Facultad de Medicina.

María Clara Echeverry, Universidad Nacional de Colombia

Universidad Nacional de Colombia. Carrera 30 # 45 - 03. Bogotá. Colombia. 57 (1) 3165405. Profesora Asociada. Departamento de Salud Pública. Facultad de Medicina.

Análida Elizabeth Pinilla, Universidad Nacional de Colombia.

Residencia:

Calle 90 #49 A- 44  Torre 2 Apto 301. Paseo de la Castellana. Bogotá. Colombia

Departamento de Medicina Interna. Profesora Asociada.

Publicado

2012-03-23

Cómo citar

López, O. Y., López, M. C., Corredor, V., Echeverry, M. C., & Pinilla, A. E. (2012). Diferenciación del complejo Entamoeba histolytica/Entamoeba dispar mediante GAL/GALNAC-LECTINA y PCR en aislamientos colombianos. Revista Médica De Chile, 140(4). Recuperado a partir de https://mail.revistamedicadechile.cl/index.php/rmedica/article/view/1474

Número

Sección

Artículos de Investigación